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Wednesday September 13, 2006 Questions for Wildwater:
1) What exactly are "lox" recombination sites?
2) Their interpretation was that SCR has opposing effects in relation to RBR function and activity--what might this mechanism look like?
3) Where is the proximal meristem? What makes proximal meristematic cells different from other meristems in the root? Does the lack of effect on proximal meristems by rBRr imply that these cells are maintained by a separate pathway?
If they used Dex to try and restore root growth in
scr-4 rRBr plants and it did not work, would this be
because RBR gene is downstream of SCR?
Why did they use scr-4?
I am a little confused. Was root growth reduced by RBR
overexpression (1343) and by the scr-4 rRBr mutant as
well?
In the scr-4, rBRr double mutant there's a restoration of the QC along with additional columella cells but there is no restoration of root size. I'm not sure how this is possible? I would guess that scr is also required for QC maintenance?
In page 1342 top second column, they claim that there is a suggestion that QC cells devide more frequently because ~30% of the rRBr plants showed expression, which I do not find really convincing. But is there really any reason to expect more divisions in these cells (rRBr)?
This is somewhat in relation to Cat's previous questions, it's hard to say if IAA differences are actually playing a role here, especially since there was no difference in root length in relation to the mutants. Is this reflecting more of an energy limitation?
-Ramon
Question 1) In the model on page 1345 the authors show a pathway for RBR pathway action and interaction in plants, however I feel that this model is a bit premature, is there any other evidence in plant systems to support this model?
Question 2) The authors have based the idea that the stem cells are maintained further away from the QC due to the action of RBR, however they state that there is a difference in the final size of the differentiated cells, could this effect be due to IAA maximum?
Question 3) I am not convinced that the RBR regulating the Stem cells is the entire story, how does IAA fit into the picture?
Cat