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Monday October 02, 2006
comment to Ramon on Mallory Ramons wrote:
1) On the Mallory paper, I am still not sure how the transformation of
wild types were able to exhibit mutant phenotype. Should the wild type
copy be able to overcome the mutant phenotype?
The wild type would not be expected to overcome...
in response to Christina RE: Mallory 2005 Christina asked: Why did they not do a protein blot? (figure 5) just because there is no cleavage
does not mean that there is protein being produced. The miRNA might bind weakly
preventing translation to occur.
Good point at least in...
response to Ramon regarding Short root. I guess that the evidence for SHR causing the assymetric cell division is that it is missing in the mutant and they seem to be able to induce assymetric cell divisions in the pSHR:SHR:gr construct plants that are not seen in the controls.What genes would be...
Thursday September 28, 2006
Mallory, Vazquez 1) On the Mallory paper, I am still not sure how the transformation of wild types were able to exhibit mutant phenotype. Should the wild type copy be able to overcome the mutant phenotype?
2) On the Vazquez paper, why is silencing shown as seperate...
Wednesday September 27, 2006
Christina's questions 9/28/06
? Mallory 2005
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figure 1, how can we know for sure that the miR160 is THE miRNA that is causing
cleavage these ARF RN...
Monday September 25, 2006
ok, let's add some comments Going back to the Levesque et al (2006) paper. I am still not sure how they were able to discern asymmetric cell divisions (fig 2J). I just feel the authors are overextrapolating, or state it as "matter of fact." I agree that SHR has a role in cell division....
Thursday September 21, 2006
C'mon everyone, post! Let's have a comment frenzy! The blog is kind of boring if we don't actually use it.
I mean, I know I'm mostly just putting off studying for my biochem exam, but....
Questions 9/21/06 Cat 1) Pertaining to Surge and Destroy: Does the lack of lethal mutants with single mutations in the PIN proteins suggest that a certain amount of Auxin can be transported without PINs or that there is a compensation during embryogenesis?
2)...
Wednesday September 20, 2006
Mark's Questions 1) are there seperate sets of ARFs, AUX/IAA proteins, etc. for IAA and IBA etc? Do the same PIN proteins interact with IAA and IBA? The generalization of "auxin" is confusing...
2) I'm not sure I understand how (theoretically) the presence of ARFs...
Jenik 1.-How good or how reliable are results that came from DR5 experiments? By the way described it seems there's too much uncertainty in using DR5 as a reporter of IAA activity.
2.- Why would nph4 and nph4-like mutants exhibit deficiency of cell expansion only...
Tuesday September 19, 2006
Christina's questions 9/19/06 – Jaillais 2006
0.25in;" class="MsoNormal"> -[if" alt="" />supportLists]--> 1) In
figure 2, I don?t see much difference between n-p and q-s.
0.25in;" class="MsoNormal"> -[if"...
Wednesday September 13, 2006
9/14/06 questions, Wildwater et al. Questions for Wildwater:
1) What exactly are "lox" recombination sites?
2) Their interpretation was that SCR has opposing effects in relation to RBR function and activity--what might this mechanism look like?
3) Where is the proximal meristem? ...
Wildwater-Qs In the scr-4, rBRr double mutant there's a restoration of the QC along with additional columella cells but there is no restoration of root size. I'm not sure how this is possible? I would guess that scr is also required for QC maintenance?
In page 1342 top...
Questions for RBR Paper Question 1) In the model on page 1345 the authors show a pathway for RBR pathway action and interaction in plants, however I feel that this model is a bit premature, is there any other evidence in plant systems to support this model?
Question 2) The...
Tuesday September 12, 2006
RBR questions 9/12/06
? Wildwater et al, Cell 2005
0.25in;"> userdefined=""> supportLists]--> 1) After
showing the reduced protein expression via western blot (fig.1K), couldn‘t the
authors have fused GUS or ...
